Reporter

Part:BBa_J100314:Design

Designed by: Monica Prudencio   Group: Campbell M Lab   (2017-01-19)


actClone Red (J100204) with scrambled OmpR promoter (J100313)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 841
    Illegal SpeI site found at 868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 841
    Illegal SpeI site found at 868
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 841
    Illegal SpeI site found at 868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 841
    Illegal SpeI site found at 868
    Illegal AgeI site found at 1557
    Illegal AgeI site found at 1669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 702


Design Notes

N/A


Source

Assembled by inserting scrambled OmpR partial promoter (J100313) into actClone Red (J100204) using Golden Gate Assembly with BsaI.

References